bacterial biochemical test Flashcards

1
Q

oxidase positive

A

Pseudomonas spp
Burkhulderia
Moraxella
Aeromonas spp.
Vibrio spp
Campylobacter
Helicobacter
Actinobacillus
Capnocytophaga
HACIK (H variable)
Pasteurella spp.
Bordetella pertussis .

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2
Q

Oxidase Negative

A

Enterobacteriales
Staphylococci
Streptococci
Enterococci
Neisseria
Yersenia
Salmonella
Shigella
Stenotrophomonas
Acinetobacter

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3
Q

Catalase Negative

A

Streptococci
Enterococci
Lactobacillus
Clostridium
Staph anaerobicus

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4
Q

What are the strept milliri group?

A

Milliri group have very small colony but large zone of haemolysis:
CIA :Constalatius , Intermedius , Angenosus

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5
Q

Beta Haemolytic bacteria

A

SUAR- narrow
Corynebacteria
Listeria
Group A,B,C,G strepts
Enterococuui
Clostridium : perfrengens , tetani , Novyi

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6
Q

grows in air, may require CO2, star shaped non-haemolytic colonies with rough surface, may produce pitting of agar
– Catalase positive, Oxidase positive and Urease negative

A

Aggregatibacter actinomycetemcomitans

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7
Q

HACIK Organism :
requires 5% to 10% CO2, small moist colonies with clear centres surrounded by flat growth, non-haemolytic
– Catalase negative, oxidase positive and urease negative

A

Eikenella corrodens

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8
Q

HACIK Organism : CO2 not required, either spreading corroding colony or smooth convex colony, often produces mucoid colonies with a small zone of β-haemolysis – Catalase negative, oxidase positive and urease negative

A

Kingella Kingae species:

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9
Q

may require CO2 addition, smooth, convex and opaque colonies, slight a-haemolysis
– Catalase negative, oxidase positive and urease negative

A

Cardiobacterium

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10
Q

grows in air + CO2, yellowish non-haemolytic colonies 1.5mm at 24hr – Catalase negative, oxidase negative and urease negative

A

Aggregatibacter aphrophilus

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11
Q

-Gram-positive rod - long, branching and sometimes beaded. -Slow-growing
-dry chalky colonies which are cream, pale orange or pink.
-It is weakly AAF by Kinyoun’s stain
-Also positive by Gomori methenamine silver staining.
-Co-trimoxazole is the first line treatment

A

N. asteroides and N. brasiliensis.

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12
Q

T/F: Capnocytophaga Canimorus is Oxidase positive

A

T

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13
Q

T/F:
Stenotrophomonas is Oxidase Negative

A

T

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14
Q

T/F:
Candida albicans is urase negative and Cryptococcus neoformans rapid positive

A

T

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15
Q

Gram-negative rod, non-lactose fermenter, DNAse positive; some strains produce a bright red pigment .
-intrinsic colistin resistance , and many are AMPc producers
-carbapenems, ciprofloxacin and aminoglycosides are likely treatment options

A

Serracia marcescens

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16
Q

important examples of intrinsically colistin-resistant organisms?

A

Edwardsiella spp, Burkholderia cepacia, Proteus spp, Providencia spp, Serratia spp

17
Q

-Gram-negative
-facultative anaerobe
-nonmotile
-pleomorphic
-bipolar staining “safety pin”
- catalase +
- oxidase +
- commonly cause animal bite infection

A

Pasturella spp

18
Q

Wat are the 3 fastidious Haemophilus spp that can cause Infective endocarditis ( included in HACEK Group)?

A

1- Haemophilus parainfuenzae
2- Haemophilus aphrophilus
3- Haemophilus paraphrophilus

19
Q

What is the commonest cause of septic arthritis in infants ?

A

Haemophilus influenzae type b