XII: Chapter 12- Biotechnology and its application Flashcards

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1
Q

Give examples of lepidopterans.

A

Tobacco budworm, armyworm

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2
Q

Proteins coded by which genes control cotton bollworms?

A

Cry1Ac and Cry2Ab

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3
Q

Proteins coded by which gene control corn borers?

A

Cry1Ab

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4
Q

How were nematode specific genes introduced into the host plant?

A

Using agrobacterium vectors

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5
Q

How many therapeutics have been approved for human use worldwide and in India?

A

30 worldwide and 12 in India

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6
Q

When was genetically engineered insulin first produced?

A

1983

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7
Q

When was the first clinical gene therapy given?

A

1990

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8
Q

ADA deficiency stands for

A

Adenosine deaminase deficiency

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9
Q

As a first step towards gene therapy which cells of the ADA deficiency patient were cultured?

A

Lymphocytes

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10
Q

How was ADA cDNA introduced into cultured lymphocytes for gene therapy of ADA deficiency?

A

Using retroviral vector

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11
Q

No transgenic model exists for cystic fibrosis. True/false?

A

False. Transgenic models exist for cystic fibrosis.

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12
Q

No transgenic model exists for Alzheimer’s. True/false?

A

False. Transgenic models exist for Alzeihmer’s.

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13
Q

When was the first transgenic cow made?

A

1997

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14
Q

How many gram of protein was present per litre in the milk produced by Rosie the transgenic cow?

A

2.4g

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15
Q

GEAC stands for

A

Genetic engineering approval committee

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16
Q

In which year did an American company get patent rights on Basmati rice through the US Patent and trademark office?

A

1997

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17
Q

How many amino acids does insulin have in both of its chains?

A

A- chain : 21 amino acids

B-chain : 30 amino acids

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18
Q

What are second generation vaccines?

A

rDNA vaccines

19
Q

What are third generation vaccines?

A

DNA vaccines

20
Q

What is the use of interferon alpha therapeutic?

A

Treatment of cancer

21
Q

What is the use of tissue plasminogen activator therapeutic?

A

Myocardial infarction

22
Q

What is the use of the therapeutic DNAse?

A

Cystic fibrosis

23
Q

What is the use of the therapeutic OKT-3?

A

immunosuppressive agent to prevent acute rejection in case of organ transplant

24
Q

What is the use of the therapeutic GCSF?

A

Cancer treatment

25
Q

What is the blood tested for in direct ELISA?

A

Antigen

26
Q

What is attached to the well in direct ELISA?

A

Enzyme linked antibody

27
Q

What is the blood tested for in Indirect ELISA?

A

Antibody

28
Q

What is present on walls of wells in indirect ELISA?

A

Antigen

29
Q

What is the blood tested for in sandwich ELISA?

A

Antigen

30
Q

What is attached on the walls of wells in sandwich ELISA?

A

Primary antibody

31
Q

Which part of the insect does Bt toxin affect?

A

Epithelial cells of midgut

32
Q

Transgenic golden rice is made by the addition of which gene in Oryza sativa?

A

Beta-carotene producing gene from daffodils or carrots

33
Q

Which plant was genetically engineered to make hirudin?

A

Brassica napus

34
Q

RNA interference is present in all organisms as a method of cellular defence. True/false?

A

False.

RNA interference is present in all eukaryotes as a method of cellular defence.

35
Q

Which was the first transgenic plant available in market?

A

Flavr savr tomato

36
Q

Golden rice is rich in

A

Vitamin A and iron

37
Q

What are the qualities of flavr savr tomatoes which make them different from other tomatoes?

A

Longer shelf life, stronger taste, bigger

38
Q

What is the function of genetically modified pseudomonas florescence?

A

Prevents frost damage on the plants that it grows

39
Q

What is the super bug?

A

Pseudomonas putida

Scavenger of oil spills

40
Q

HGH insert in common carp makes it

A

2 times in size

41
Q

Name the first transgenic sheep.

A

Polly molly

42
Q

Name the first successful mammalian clone

A

Dolly the sheep

43
Q

To create first transgenic sheep, the host was

A

Black faced ewe

44
Q

To create first transgenic sheep the host was

A

White faced ewe