working with cells

Question 1

4 are are 4 universal conditions for cell cultureq?

Answer 1

aspetic techniques
temperature
atmosphere
nutrients

Question 2

what are the two species of yeast most commonly used in research

Answer 2

pombe- fission

cerevisiae- budding

Question 3

what are haloferax

Answer 3

arhcae that are model organism

grow at 45 dgrees in hihg salt

Question 4

what are 4 examples of cell culture from multi-cellular organisms

Answer 4

fibroblasts
myoblasts
oligodendrocytes
liquid cultures for mammalian cells

Question 5

2 key features for growing cultures in a lab

Answer 5

physiological temp and pH

grow on flat surface

Question 6

what are HeLa cells

Answer 6

immortal cells of henrietta lacks

ethical debate

Question 7

how is a cell line established

Answer 7

isolate single cells from tissue by dissection, mincing , enzymatic dissociation

Question 8

how are transformed (immortal) cell lines established

Answer 8

same as cell line but cells taken from tumour

express viral proteins and genes that confer immortality

Question 9

what are 3 types of organotypic cultures

Answer 9

primary cell culture
tissue slices
embryonic stem cell organoids