Virology

Question 1

What is a virus?

Answer 1

-infectious, obligate intracellular parasite
-not always smaller than the bacteria but too small to be observed with microscopes
-

Question 2

T/F
You can see viruses inside the cell when they infect the host cell

Answer 2

False
We can see the components of the viruses but not directly viruses.

Question 3

Replication of viruses

Answer 3

First phase= no replication
Second phase= burst or yield happens one virus make thousands

Question 4

Susceptible cell?

Answer 4

Has a functional receptor for a given virus-the cell can or cannot be cable to support viral replication.

Question 5

Resistant cell

Answer 5

Cell Has no receptor for the virus.

Question 6

Permissive cell

Answer 6

The cell has the capacity to replicate virus- it can be susceptible or not meaning it does/ doesn’t receptor in the cell membrane.
Only in the susceptible and permissive cells can take up a virus particle and replicate it.

Question 7

What is infectivity?

Answer 7

Viruses ability to infect other cells or organism, spreading ability.

Question 8

Plaque assay to measure infectivity

Answer 8

A plaque assay is a laboratory technique used to measure the number of infectious viral particles present in a sample. It involves infecting a monolayer of susceptible host cells with the virus of interest, allowing the virus to replicate and spread, and then staining the cells to visualize the areas of cell death, or plaques, caused by viral infection. Each plaque represents a single infectious viral particle that was able to successfully infect a host cell. By counting the number of plaques formed, scientists can determine the concentration, or titer, of infectious virus in the original sample. This method is commonly used in virology research to quantify viral infectivity and assess the efficacy of antiviral treatments.

Question 9

Hemagglutination- measurement of virus particles?

Answer 9

We are using susceptible but not permissive RBCs. If it forms agglutination= there are no virus
If not it is infected by the virus

Question 10

ELISA- enzyme linked immunosorbent assay

Answer 10

Detecting viral antigens with IgG with indicators such as(phosphor or etc.)