Vectors Flashcards
where are Bacterial Cloning plasmids derived from? one example
double-stranded circular DNA found in bacterial cells
E.G pUC
what three things are bacterial cloning plasmids used for?
cloning small gene inserts
sub-cloning
downstream manipulation
what size fragment can be cloned in a bacterial cloning plasmid?
small
< 10kb
what are bacterial expression pladmids used for
cloned segments of DNA to be translated into protein inside a bacterial plasmid
example of bacterial expression plasmid
pET
what size fragments can be produced from bacterial expression plasmids?
large amounts
characteristic of shuttle vectors
can replicate in two different organisms
use of yeast plasmids
transmission of DNA sequences in yeast
structure of yeast artificial chromosomes (YACs)
linear DNA vectors
what do YACs contain
telomere, centromere and selective marker gene sequences
what size DNA segment can YACs carry?
100kb to 1Mb
what are bacterial artificial chromosomes based on
F plasmids
what do BACs contain
bacterial Ori and an antibiotic resistance gene for plasmid selection in E.coli
what size fragment can BACs carry?
up to 300kb
what are PACs derived from?
P1 phage
