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Diagnostic Microbiology > Urinary Tract Infections > Flashcards

Urinary Tract Infections Flashcards

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Biology 101 flashcards
1
Q

where does the renal artery enter and renal vein and ureter exit

A

hilum

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2
Q

what is cystitis?

A

inflammation of the bladder

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3
Q

what are most common UTI pathogens?

A

E coli, s saprophyticus, ps aeruginosa, proteus klebsiella,, s aureus

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4
Q

how many WBC in normal urine sample?

A

<10 wbc/cmm

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5
Q

what are casts composed of?

A

cylindrical mucoprotein

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6
Q

what agar is used for urine analysis?

A

CLED

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7
Q

what does CLED stand for?

A

cysteine lactose electrolyte deficient medium

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8
Q

is CLED selective or differential?

A

differential

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9
Q

what does electrolyte deficient media do?

A

prevent proteus swarming

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10
Q

what indicator is in CLED?

A

Bromophenol blue

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11
Q

what colour to bromophenol blue chan in lactose fermentation?

A

yellow - acid production

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12
Q

what will grow yellow on CLED?

A

coliforms - lactose fermenters
GNB - e coli, enterobacter, klebsiella
GPos -s aureus, s sapro, entero, strep

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13
Q

what will grow blue on CLED?

A

non lactose fermenters

p aeruginosa, mirabolis

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14
Q

what test in used on non lactose fermenting GNB?

A

oxidase test

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15
Q

what is carried out on samples over 50 WBC?

A

first line AST testing

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16
Q

why would epithelial cells be seen in a sample

A

non mid stream

17
Q

how is cast investigation carried out?

A

centrifuged, fast red counting chamber, dim microscope

18
Q

what are the types of cast

A

hyaline, red cell, granular, WBC

19
Q

what are normal crystals

A

ca oxalate, ca phosphate, triple phosphate, ca carbonate

20
Q

what are abnormal crystals?

A

cholesterol, bilirubin, cysteine, leucine, tyrosine, sulfa

21
Q

what type of AST method is used on UTI?

A

Kirby Bauer

22
Q

what is kirby bauer AST?

A

make 0.5 McFarland standard
lawn of growth
place antibiotic disc, EUCAST guidelines

Diagnostic Microbiology (2 decks)

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