Translation (BIO)

Question 1

contains codons that code for the peptide sequence.

Answer 1

mRNA

Question 2

contains the anticodon on the “tip” and the corresponding amino acid on the “tail”. Link the correct amino acid to its corresponding mRNA codon through codon-anticodon interaction.

Answer 2

tRNA

Question 3

forms the ribosome. Catalyzes the formation of the peptide bond.

Answer 3

rRNA

Question 4

is the enzyme that catalyzes protein synthesis.

has 2 subunits - the large and the small.

The large subunit is responsible for the peptidyl transfer reaction.
The small subunit is responsible for the recognizing mRNA and binds to the Shine-Dalgarno sequence on the mRNA (Kozak sequence for eukaryotes).
Both subunits are needed for translation to occur and they come together in a hamburger fashion that sandwiches the mRNA and tRNAs in between.

Answer 4

Ribosomes

Question 5

A technique used to analyze protein post translational modifications (PTM)
Most commonly used to detect carbohydrate epitopes

Answer 5

Eastern blotting

Question 6

Used for the detection of a specific DNA sequence in a DNA sample by transferring electrophoresis-separated DNA fragments to a filter membrane and then to fragment detection by probe hybridization

Answer 6

Southern blotting

Question 7

An adaptation of the Southern Blot used to detect specific sequences of RNA by hybridization with complementary DNA

Answer 7

Northern blotting

Question 8

A technique used to detect specific proteins in a sample of tissue

Answer 8

Western blotting