Topic 5 (Respiration)

Question 1

What is the definition of oxidation?

Answer 1

loss of e-s or H2

Question 2

What is the definition of reduction?

Answer 2

gain of e-s or H2

Question 3

What is cristae?

Answer 3

fold of inner membrane in mitochondria

Question 4

Draw a labelled diagram of mitochondria (should include 7 labels)

Answer 4

• cristae
• matrix
• inner membrane
• outer membrane
• intermembrane space
• ribosomes
• mitochondrial DNA

Question 5

What are the 4 main stages of aerobic respiration?

Answer 5

1. glycolysis
2. Link reaction
3. Kreb’s cycle
4. Oxidative phosphorylation

Question 6

What does Pi stand for?

Answer 6

Inorganic phosphate

Question 7

what is glycolysis?

Answer 7

lysis of glucose

Question 8

where does glycolysis take place?

Answer 8

cytoplasm

Question 9

what are the products of glycolysis?

Answer 9

2x Pyruvate (3C) molecules
Net gain 2 ATP
2 reduced NAD

Question 10

Why is the net gain of 2 ATP in glycolysis?

Answer 10

2 ATP used in phosphorylation
4 ATP made in oxidation/dephosphorolation

Question 11

What are the steps of glycolysis?

Answer 11

Glucose (6C) → 2x Glycerate-3-phosphate
while 2 ATP → 2 (ADP +Pi)
Glycerate-3-phosphate → 2x pyruvate (3C)
while 4 (ADP+Pi) → 4ATP
and 2NAD + e- + H+ → Reduced NAD

Question 12

What is used for link reaction?

Answer 12

Pyruvate (+ coenzyme A)

Question 13

What is the product of link reaction?

Answer 13

Acetyl-CoA

Question 14

How many ATPs are made in total using one glucose in respiration?

Answer 14

38

Question 15

Draw glycolysis

Question 16

Draw link reaction

Question 17

Draw krebs cycle

Question 18

Draw oxidative phosphorylation

Question 19

What is the whole point of respiration

Answer 19

Produce as much energy possible in the form of ATP

Question 20

Breaking
ATP ->
ADP ->

Answer 20

ATP -> ADP + Pi
(Di-phosphate)
ADP -> AMP + Pi
(Mono phosphate)
Hydrolyse using water

Question 21

What are 2 ways of ATP production

Answer 21

1. Substrate level phosphorylation
2. Oxidative phosphorylation

Question 22

4 steps of aerobic respiration and where they occur

Answer 22

1. Glycolysis - cytoplasm
2. Link reaction - matrix
3. Krebs cycle - matrix
4. Oxidative phosphorylation - inner membrane

Question 23

What is substrate level phosphorylation?

Answer 23

Simply
ADP + Pi -> ATP

Question 24

How many ATPs do oxidation and substrate level phosphorylation make?

Answer 24

34
4
Respectively

Question 25

Oxygen acts as… in respiration

Answer 25

FINAL ELECTRON ACCEPTOR