Structure, Growth, Media, Stains

Question 1

Compoenents fo Gram +

Answer 1

1. Teichoic Acid in cell wall (adhesion)
2. Thick peptidoglycan wall (protection from osmotic damge)
3. Purple Gram Stain

Question 2

Components of Gram -

Answer 2

1. LPS in the outer membrane (Lipid A (toxic), O Ag,)
2. Thin peptidoglycan
3. Periplasmic Space (storage space b/w IM & OM, where enzymes break down large molecules, B-lactamases)
4. Pink on gram stain

Question 3

Acid Fast Components

Answer 3

1. Mycolic Acid in cell wall
2. Stains Red w/ acid fast stain

Question 4

What type of bacteria produce endospores?

Answer 4

Gram + ONLY (Bacillus, Clostridium)

Resistance to heat, chemicals, & dehydration

Question 5

Lag Phase of Bacterial Growth

Answer 5

Cells are not actively dividing

Turning on enzymes and gathering materials it needs to grow

Question 6

Log Phase of Bacterial Growth

Answer 6

This is whe the bacteria are most susceptible to abx tx

exponential growth of bacteria

Question 7

Selective Medium for culture

Answer 7

A selective medium selects fof certain bacteria by the inclusion of special nutrients/abx

Question 8

Differential medium for culture

Answer 8

A differential medium differentiates bacteria based on colonial morphology or color.

Question 9

Medium anaerobes grow on

Answer 9

Thioglycolate

Question 10

Medium Legionella grows on

Answer 10

Charcoal-yeast extract agar (CYE agar)

selective

Question 11

Medium Vibrio cholerae grows on

Answer 11

Thiosulfate Citrate Bile Salts Sucrose agar (TCBS)

orange color

Likes alkaline growth medium

Question 12

Medium Neisseria from normally sterile sites & Haemophius grows from

Answer 12

Chocolate agar

Question 13

Medium Neisseria grows on from sites w/ normal flora

Answer 13

Thayer-Martin

Selective

Question 14

Growth requirements for Haemophilus

Answer 14

X (protoporphyrin)

V (NAD)

Question 15

Obligate aerobes

Answer 15

Have no fermentation pathways

Produce Superoxide dismutase

Mycobacertium, Pseudomonas

Question 16

Microaerophilic

Answer 16

Campylobacter

Helicobacter

Question 17

Faculative Anaerobes

Answer 17

Most microbes fall under this class

Ferment when O2 is depleted

All enteric bacterias fall under this class

Question 18

Obligate anaerobes

Answer 18

Lack superoxide dismutase

Lack catalse

Fermenters

Cannot use O2 as terminal e- acceptor

ABC (Actinomyces, Bacteroides, Clostridium)