Smearing

Question 1

SMEARING METHOD:

_______: for BM prep and not for PBS

1. aka
2. Adv:
3. Disadv:

_____: method that is uncommonly performed
1. aka

Answer 1

COVERSLIP METHOD

1. EHRLICH
2. superior leukocyte distribution
3. more technically difficult

GLASS SLIDE-COVERSLIP METHOD
1. BEACOM

Question 2

SMEARING METHOD:

_______: most convenient and most commonly used

1. aka
2. size of the blood
3. distance from the end of the slides to another end should be at least
4. when we positioned it we maintained an angle
   between _____° (Henry), _____° (Steninger)
5. • more viscous blood = ____ angle
   • less viscous blood = ____ angle
6. adv: easier to find _____
7. we might see broken cell or smudged cells aka ____ which are actually ____ that are fragile
   - commonly seen in ______

Answer 2

TWO-GLASS SLIDE METHOD

1. WEDGE SLIDE/ PUSH SMEAR
2. 2-4 mm
3. 1 cm
4. 30-45° (Henry), 25-40° (Steninger)
5. • LOWER angle
   • HIGHER angle
6. ABNORMAL CELLS
7. SQUASH CELLS; LYMPHOCYTES; LYMPHOCYTIC LEUKEMIA

Question 3

SPREADER SLIDE:

1. must be ____ than stationary slide
2. ______ are often located at the two sides of the slides, while the ______ are at the center
3. presence of dirty or clotted blood may result to. ______
4. w/ ____ edge

Answer 3

1. narrower
2. IMMATURE/LARGE; SMALLER
3. gritty appearance
4. beveled edge

Question 4

AUTOMATED SMEAR:

_____: precision blood spreader prepares dual
smears simultaneously at a constant angle and speed. But spreader here is not another slide, it is already part of the device.

1. utilize the ____ procedure
2. it is ____ only
3. allows us to prepare __ slides simultaneously at a constant single speed

Answer 4

MINIPREP/ HEMAPREP

1. wedge
2. semi-automated only
3. 2

Question 5

AUTOMATED SMEAR:

_____: instrument spins causing the slide to be covered with a monolayer of cells beam of sensor light to detect blood distribution

1. more ____
2. uses ____ to detect 5e filling
3. type of smear that we produced here is called ___

Answer 5

HEMASPINNER

1. AUTOMATED
2. BEAM OF SENSOR LIGHT
3. SPUN SMEAR

Question 6

_____: used in wedge method; considered a concentrated smear of wbcs

1. finding ____ or ____ that are present in small numbers
2. for easier location of ___ & _____
3. recommended when the wbc count is ______
4. only around ___wbc may be seen in emmersion field, it in Buffy coat smear we see a lot

Answer 6

BUFFY COAT SMEAR

1. reactive /immature or abnormal cells
2. bacteria & parasites
3. <1x10^9
4. 3-5 wbc

Question 7

THICK SMEAR: WET

1. place a large drop of blood together with ____ to emulsify and cover it with coverslip
2. used to observe _____

THICK SMEAR: DRY
1. prepare in diagnosis of ____ together with thin smear.
2. after drying, immerse in ____
- then stain with ______
- purpose:

THIN SMEAR: DRY
1. after drying, do not immerse with water but fix with ______
2. purpose:

Answer 7

THICK SMEAR: WET

1. NSS
2. motility of the parasite

THICK SMEAR: DRY
1. Trypanosmomes
2. DISTILLED WATER
- WRIGHT/ GIEMSA STAIN
- SCREENING OF PARASITIC INFECTION

THIN SMEAR: DRY
1. METHANOL
2. SPECIE IDENTIFICATION

Question 8

CHARACTERISTICS OF A PROPERLY PREPARED WEDGE SMEAR

1. must occupy at least ____ to ___ of the slide
2. should terminate in _____
3. should contain atleast ____ in which ____% of RBCs do not overlap

Answer 8

1. 1/2 to 2/3
2. FEATHERY TAIL
3. 10 LPF; 50%

Question 9

CAUSES OF THICK SMEAR

1. too large drop of blood: greater than ___ will result to thick smear
2. too ___ spread: spreading should be rapid and smooth only
3. too high angle: requires angle is _____
4. high ____
   - remedy: add ____ prior to smearing
5. RBCs form _____

Answer 9

1. 2-3mm
2. FAST
3. 25-45
4. HEMATOCRIT
   - NSS
5. ROULEAUX

Question 10

CAUSES OF THIN SMEAR:

1. too small drop of blood: less than __ is inadequate
2. too ____ spread
3. too low angle: lower than __ is inadequate will cause very long and thin
4. low ___ = with higher plasma volume and lesser viscosity

Answer 10

1. 2mm
2. slow
3. 25 degrees
4. LOW HCT

Question 11

CAUSES OF GRITTY APPEARANCE

1. accumulation of ___
2. _____ causes blood to clump, causes a ___ background

Answer 11

1. nucleated cells
2. HEPARIN; dark

Question 12

ARTIFACTS OF BLOOD SMEAR PREPARATION

1. Blood Sample That Can Be Used in Smear Preparation (2)
   - ___ has more volume and may also delay the prep but should not be longer than ___hrs

EDTA
2. ___ vacuolation
3. reactive lymphocytes causes ____ appearance with convoluted nuclei
4. RBC speculation become _____, borders are no longer smooth as it has already spicules.
5. ___ may appear
6. ____ indicates abnormal RNA metabolism, but in artifact it is just an artifact within clin sig
7. drying artifacts
- ___ environment
- severe
- water contamination of fixative causes _____
- RBC has ___ appearance where there’s hairy appearance of lymphocytes

Answer 12

1. FRESH CAPILLARY BLOOD & EDTA ANTICOAGULATED BLOOD SAMPLE
   - EDTA; 2-3 hrs

EDTA
2. Monocyte
3. swiss cheese
4. echinocytic
5. Dohle bodies
6. Basophilic granules
7. drying artifacts
- HUMID
- ANEMIA
- RED CELL ARTIFACTS
- moth-eaten

Question 13

METHODS OF DRYING BLOOD SMEAR:

1. ______ (fan)
2. use of _____
3. use of ______
4. When blowing air unto the blood smear, we also introducing moisture from our breath, moisture will cause an artifact

Answer 13

1. Air drying
2. low flame
3. oven
4. echinocytic

Question 14

preparation of blood smear:

1. pure and cells are seen as they are; but, blood will immediately clot and there’s limited volume which can prepare only 1-2 slides
2. allows multiple smears;
   allows delays (should be prepared within 3hrs from collection; beyond 5hrs
   - RBCs will become _______
   - WBCs will become _______
   - Neutrophils will be _____

Answer 14

1. fresh capillary blood
2. EDTA-anticoagulated venous blood
   - echinocytic
   - necrobiotic
   - vacuolated