Roundup

Question 1

What are good targets for herbicides?

Answer 1

Pathways leading to essential amino acids

Question 2

Glyphosate (Roundup)

Answer 2

an inhibitor of EPSP synthase

Question 3

EPSP synthase

Answer 3

an enzyme of the shikimic acid pathway that blocks the production of aromatic amino acids

Question 4

What do “Roundup Ready” plants carry?

Answer 4

A bacterial gene for EPSP synthase that was introduced into plants using Agrobacterium-mediated transformation

Question 5

What kind of gene was introduced to “Roundup Ready” plants?

Answer 5

A gene that encodes a protein that is resistant to glyphosate

Question 6

What are the three types of reversible inhibitors?

Answer 6

1. Competitive inhibitors
2. Non-competitive inhibitors
3. Un-competitive inihibtoors

Question 7

Competitive inhibitors

Answer 7

Inhibitor mimics substrate and fits into the active site

Question 8

How can competitive inhibitors be identified?

Answer 8

the kinetics of their inhibition

Question 9

In the presence of a competitive inhibitor, KM _______, and Vmax _________.

Answer 9

increases; stays the same

Question 10

The effects of competitive inhibition can be overcome by doing what?

Answer 10

increasing [S]