root tip squash

Question 1

Method

Answer 1

• cut root tip with scalpel
• transfer tip into HCL and wait 5 minutes
• remove from HCL and wash with distilled water
• remove very tip with scalpel
• tip onto slide, add stain so that chromosomes are visible
• ## lower cover slip carefully

Question 2

why do we squash root tip downwards?

Answer 2

To create single layer of cells to allow light to pass through, sidewards would cause rolling over and chromosomes to break