Recombinant Proteins Flashcards
1
Q
what are some hosts of RPE other than E. Coli?
A
bacteria, plants, funghi, animal and human cell lines
2
Q
why may human cell lines be best for RPE?
A
most capable of post translational modification of RPs
3
Q
what is a drawback of using E. Coli?
A
cannot perform post translational modification - not encoded
4
Q
what is a recombinant gene often linked to so it can be expressed in E Coli?
A
linked to a promoter and recombinant gene cloned in vector
5
Q
what are transcription and translation driven by?
A
transcription driven by promoter bound to RNA polymerase
translation driven by ribosome binding (recombinant gene linked to promoter and RBS)
6
Q
what are the therapeutic uses of recombinant proteins?
A
hormones, cytokines, vaccines, monoclonal antibodies
1/3 are produced in E. Coli
