Recombinant DNA technology Lecture 3 and 4

Question 1

what are restriction enzymes

Answer 1

Restriction enzymes are an important tool to investigate and manipulate genomic DNA

Question 2

Recombinant DNA technology involves…

Answer 2

using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest

Question 3

’DNA codes for….

Answer 3

RNA codes for protein’’

Question 4

TransCription followed by….

Answer 4

transLation

Question 5

In mRNA, ‘’the introns are spliced out’’ and….

Answer 5

’the exons are expressed’’ as protein

Question 6

what can a mutation in a gene can result in

Answer 6

abnormal protein or possibly no protein

Question 7

how many genes are in each chromosome

Answer 7

100s to 1,000s of genes

Question 8

how many nucleotides are distributed over 24 chromosomes

Answer 8

3.2 x 109 nucleotides

Question 9

how much of the human genome do the coding exons

Answer 9

~1.5%

Question 10

what gene mutation causes cystic fibrosis

Answer 10

Cystic fibrosis is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene for example the delta 508 single base deletion