Recombinant DNA techniques Flashcards
what technique would you use for detection of repeats found in Huntington’s and fragile X?
PCR
What technique would you use for detection of an infection agent?
PCR
If you wanted to detect a mutation that disrupts a restriction site, such as in Sickle cell-this DNA using technique would be best
southern blot
Not to be confused with PCR, you would use this if you wanted to detect the presence of a specific antigen due to infection
ELISA
If you were to do a comparison of multiple gene expressions, for example comparing two strains of cancers
Micro array
to identify a truncated protein you would want to use?
Western Blot
to detect a disease specific protein marker such as troponin you would use?
ELISA
to amplify a protein coding sequence
RT-PCR
to identify a specific sequence within a genome ? * this is the best technique you could possibly use for accuracy*
Sequencing
what technique would be used for detection of specific transcripts
RT PCR
which technique would help to identify the Philadelphia chromosome found is CLL chronic lymphocyte leukemia?
Hybridization: FISH
to detect a VNTR variable number of tandem repeats such as Huntington’s and fragile X you could use PCR and what else?
restriction enzymes
using a southern blot to detect sickle cell, what are you trying to detect?
RFLP ( restriction fragment length polymorphism)
