Quiz on 5/10 Flashcards
What does the Oxidase slant test for?
Cytochrome oxidase (enzyme)
What does a positive Oxidase slant test look lie?
Dark purple color change withint 20sec
What tool does the Oxidase slant use?
loop
What does the Brilliant green agar test for?
fermentation of lactose and sucrose
Which bacteria does the Brilliant green agar select for?
select for Gram - using green dye
what tool is used for the Brilliant green agar test?
cotton swab using the zag method
If the organisim in the Brilliant green agar test ferments sugars, the ph will..
go down (acidic / fermenter)
If the organism in the Brilliant green agar test preferes peptides the pH wil..
go up (alkaline / non-fermenter)
If the test result is acidic in the Brilliant green agar test, what does it look like?
Lime green color change with no growth
What does it look like in the Brilliant green agar test if its Alkaline?
Bright pink color change with growth
What does it look like in the Brilliant green agar test if its dead?
Inhibited by green dye; plate stays orange
What does the Hekton Agar test for?
Fermentation of 3 carbohydrates: lactose, sucrose, salicin
What tool is used for the Hekton Agar test?
cotton swab using the zag method
What does the selective media composed of in the Hekton Agar test?
contains bile
this will inhibit some Gram - organisims
What is the outcome of the Hekton Agar test if its acidic?
- Fermentor
- pH goes down
- Orange color change showing growth
