Processes that sustain life: Microscopes Flashcards

Test review

1
Q

What is the depth of field

A

The visual range that is in focus from foreground to background. If a specimen is too thick, only a portion of it will be in focus at one time. Low power shows the greatest depth, in high power you will only see one layer at a time as you focus up and down.

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2
Q

What is resolution

A

The ability of the microscope to show details. High resolution has sharp, easily distinguished details. Low resolution is blurry. The ability to distinguish between things that are close together

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3
Q

What is the field of view

A

The circular area you can see when you look through the microscope. Higher magnification = smaller field of view

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4
Q

What is total magnification

A

Eyepiece magniciation multiplied by eyepiece magnification

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5
Q

What is magnification

A

The number of times larger the image you observe is compared to the actual object. 400x means 400 times larger

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6
Q

How to handle a microscope safely?

A

-hold with two hands, one on the arm one on the base
-avoid tripping on the electrical cord
-do not touch lens surface with your fingers
-only clean lens surface with lens tissue
-do not adjust focus knobs until ready to use microscope
-always focus using coarse adjustment knob first, with low power objective lens in position
-do not use coarse adjustment knob with medium or high power objective lens
-always store microscope in low power
-never tug on electric cord
-do not use wet hands around electricity

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7
Q

What was the paradigm shift from the recent happenings in science at the time

A

As much happened paving the way for modern cell theory, people began to think of the world as more of a palce inhabited by microorganisms and got a better understanding of basic life processes at a celluar level

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