Practical: Enzyme Activity Flashcards
1
Q
What enzyme is used to break down starch to maltose?
A
Amylase
2
Q
What solution is used in this practical?
A
Iodine solution
3
Q
What colour will the iodine turn if starch is present?
A
blue-black
4
Q
How much of the amylase solution, buffer solution and the starch solution will you add to the boiling tube?
A
3cm cubed of amylase
1cm squared of buffer
3cm cubed of starch (use a different syringe)
5
Q
What do you place the boiling tube in?
A
Some water of a temperature of 35 degrees celsius.
6
Q
How often do you add a drop to the spotting tile of the solution from the boiling tube?
A
Every ten seconds
7
Q
How can you calculate the rate of reaction for this experiment?
A
Rate= 1000/ time
8
Q
What are the units used?
A
s to the negative 1
