PCR and Master mix

Question 1

PCR Definition

Answer 1

Applying heat to copy the interferon Alpha gene and make multiple copies

Question 2

PCR Steps

Answer 2

Denaturation
Annealing
Extension

Repeat stages 5 times

Question 3

Denaturation

Answer 3

Attempt to denature it
Attempt to break double strand

At 94*C

Question 4

Annealing

Answer 4

Primer is annealed to leading strand

At 60*C

Question 5

Stock primer

Answer 5

Concentration of primer (x10)

Question 6

Extension

Answer 6

Make several copies
In PCR tube = DNA and Primer

At 72*C

Question 7

Working primer

Answer 7

multiply stock primer volume by 10 and add deionized water

Question 8

Master Mix

Answer 8

-dNTpis (ATCG)
-Cofactor Mg+
-Buffer
-Polymerase