Oxidative Phosphorylation (Pt. 1)

Question 1

Where does oxidative phosphorylation occur

Answer 1

Mitochondrial matrix

Question 2

How many atp are generated in oxidative phosphoryloation

Answer 2

28/32 ATP

Question 3

What is the overall net reaction

Answer 3

2NADH + 2H+ + 5ADP + 5Pi + O2 –> 5ATP + H2O = 2NAD+

Question 4

What are the key enzymes in Oxidative Phosphorylation

Answer 4

COmplex I, III, and IV

Question 5

What is ubiquinone (Q)

Answer 5

It is a small hydrohpobic electron carrier to complex III

Question 6

What is cytochrome C

Answer 6

small protein that carries electrons from III –> IV

Question 7

What are the three steps in complex I

Answer 7

1. NADH transfers 2e- to FMN
2. 2E- transferred from carrier to carrier
3. 2e- and 2H+ bind to Q, forming QH2

Question 8

What happens in complex II

Answer 8

transfers a pair of electrons to FAD –> FADH2, oxidizes succinate, reduces fumarate

Question 9

What happens in complex III

Answer 9

Has 2 Q binding sites and servar prostetic groups taht function as electron carriers

Question 10

What happens in complex IV

Answer 10

Oxidation of cytochrome C from inter membrane space to reduce oxygen to create water, 2H+ are transferred from matrix to inter membrane space