Oxidative Phosphorilation Flashcards
Where does oxidative phosphorylation take place and what is oxidised?
It takes place in the Mitochondria
NADH and FADH2 are oxidised
How are the electrons transported from NADH and FADH2 to O2?
Electron transport chain
What are the three large protein complexes that electrons from NADH+ are transported to O2 from?
NADH- Q oxidoreductase
Q- cytochrome C oxidoreductase
Cytochrome C oxidase
What happens in the first complex?
NADH uses NADH Q oxidoreductase to turn into Q
What happens in the second complex?
Q uses Q-cytochrome C oxidoreductase to turn into CYT C
What happens in the third complex?
Cyt C uses cytochrome C oxidase to turn into O2
What molecule does I-III use that diffuses in the membrane?
Ubiquinone
What small soluble protein does III-IV use
Cytochrome C
What does the F1 subunit do and what shapes does its subunits arrange themselves into?
It protrudes into the mitochondrial matrix and contains the catalytic activity
The a and B subunits are in a ring shape, the Y and E make up a central stalk
What does the FO subunit do?
It is a hydrophobic segment that spans the membrane, it contains a channel where proteins flow
How are FO and F1 connected?
They’re connected by the Y stalk and by an exterior column
How does ATP synthesis work?
ATP is bound to the a subunits, the B subunits interact with the y subunits and perform three steps to synthesise ATP
What are the three steps of ATP?
1) ADP + Pi binding ( L conformation)
2) ATP synthesis (T conformation)
3) ATP release ( O conformation)
