Nucleic Acids

Question 1

What is a nuclease?

Answer 1

just the base

Question 2

What is a nucleoside?

Answer 2

base attached to a sugar

Question 3

What kind of linkage is between the base and a sugar?

Answer 3

beta-N-glycoside

Question 4

What is a nucleotide?

Answer 4

base, sugar and at least one phosphate

Question 5

What is a nucleic acid?

Answer 5

phosphate dieter of nucleotides

Question 6

What is pi-stacking?

Answer 6

the way bases stack in DNA

Question 7

What are 3 types of interactions of other molecules with DNA?

Answer 7

selectively binding molecules ie leucine zippers
intercalation
interactions with negatively-charged backbone

Question 8

In capillary gel electrophoresis which end are the DNA strands excited with a laser at?

Answer 8

the anode

Question 9

What wavelengths are considered to be UV-C?

Answer 9

180-290nm

deadly

Question 10

What wavelengths are considered to be UV-B?

Answer 10

290-320nm

lethal/mutagenic

Question 11

What wavelengths are considered to be UV-A?

Answer 11

320-400nm

some DNA damage

Question 12

What does the ozone layer absorb?

Answer 12

All UV-C, some UV-B and no UV-A

Question 13

What reaction results in thiamine dimers?

Answer 13

photocyloaddition reaction

Question 14

What is the Maillard reaction?

Answer 14

under high temperatures carbs and AAs can react together and form carcinogens