NON-ENZYMATIC CYTOCHEMISTRY Flashcards
1
Q
- stains acid mucopolysaccharides from metachromatic colored complex
- makes use of mota’s fixative
A
toluidine blue
2
Q
what diseases does toluidine blue identifies
A
mast cell diseases
chronic basophilic leukemia
2
Q
toluidine blue results in nuclei and metachromatic granules
A
nuclei: light blue
metachromatic granules: reddish violet
2
Q
- demonstration of ferric iron
- evaluation of bone marrow storage iron
- evaluation of red cell iron utilization
- recognition of ringed sideroblasts in MDS
- diagnosis of iron overload
A
prussian blue
2
Q
prussian blue result
A
blue
3
Q
capable of reacting with aldehyde groups in: glycogen, mucoprotein, and other high molecular weight carbohydrates
A
periodic acid schiff
4
Q
PAS result
A
bright fuschia pink granules in cytoplasm
4
Q
- used for demonstrating lipids
- the reaction is stable with heat and even when the specimen is stored long
- more sensitive to primitive myeloid cells than mpo
A
sudan black b
5
Q
cells that stain negative in PAS
A
lymphocytes
erythrocyte precursors
6
Q
sudan black b result
A
black
6
Q
- identification of the phenotype of a given cell population through immunologic technique
- use of specific monoclonal or polyclonal antibodies against selected antigens
A
immunocytochemistry
6
Q
what is TdT
A
terminal deoxynucleotidyl transferase
7
Q
marker of immature lymphoid cells
A
tdt
