Microscopy

Question 1

2 ways to visualise cells using light microscopy

Answer 1

• staining (haematoxylin and eosin) > purple together
• antibodies (immunohistochemistry)

Question 1

H&E

Answer 1

• haematoxylin (blue) stains acidic structures e.g. DNA and RNA
• eosin (pink/red) stains basic structures, +ve charge e.g. protein

Question 2

immunohistochemistry

Answer 2

• antibody binds to a specific structure of a protein
• when chemical substrate is added, enzyme produces a coloured product
• signal detected when target protein is present
• can show where a protein is and how much is present