microscopes Flashcards

1
Q

light microcopes magnification

A

Light microscopes can magnify objects up to around x1,500

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2
Q

how does a light microscope work ?

A

Light microscopes magnify images of objects by using glass lenses to focus light onto or through the object

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3
Q

light microscopes resolution

A

0.2 micrometres

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4
Q

light microscope 2d or 3d images

A

2d

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5
Q

light microscope living or dead samples ?

A

living cells

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6
Q

SEM how does it work

A

by scanning a sample with a beam of electrons and collecting the signals produced to create an image

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7
Q

magnification of sem

A

can magnify objects from 10 to 500,000 times.

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8
Q

resolution of sem

A

1–2 nanometres

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9
Q

2d or 3d sem ?

A

3d

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10
Q

sem living or dead samples

A

dead samples

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11
Q

what can be seen under an SEM

A

cell membranes, endoplasmic reticulum, ribosomes, lysosomes, and mitochondria can be seen

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12
Q

what can be seen under a light microscope

A

cell walls, vacuoles, cytoplasm, chloroplasts, nucleus and cell membrane

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13
Q

how does a TEM work

A

use a beam of electrons to create high-resolution images of thin samples

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14
Q

3d or 2d images TEM

A

both

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15
Q

magnification of TEM

A

TEMs have a maximum magnification of around x1,000,000

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16
Q

resolution of TEM

A

typically less than 1 nanometre (nm)

17
Q

living or dead samples TEM

A

TEM cannot be used to visualise living material. Only thin tissues also

18
Q

what can be seen under a TEM

A

The cell wall, nucleus, vacuoles, mitochondria, endoplasmic reticulum, Golgi apparatus, and ribosomes