Methods of isolation and identification - DONE Flashcards
1
Q
Structure and chemical composition of cell wall of G (+) and G(-)
A
- G (+): thick, 50-80% peptidoglycan
* G(-): thin, 10% peptidoglycan
2
Q
Where can we find spores?
A
G (+)
3
Q
Susceptibility to disinfectants & detergents:
A
G+> G-
4
Q
DEF. FAMILY:
A
a group of related genera
5
Q
DEF. GENUS:
A
a group of related species
6
Q
DEF. SPECIES:
A
a group of related strains
7
Q
DEF. TYPE:
A
sets of strain within a species (e.g. biotypes, serotypes)
8
Q
DEF. STRAIN:
A
one line or a single isolate of a particular species
9
Q
Bacteriological examination - Direct:
A
- microscopy
- culture media
- antigen detection
- DNA detection
10
Q
Bacteriological examination - Indirect:
A
- antibodies detection
- skin test
11
Q
How are bacteria classified?
A
- staining
- shape
- respiration
- shape/ reproduction
- genus
- species
12
Q
Non cultural techniques:
A
- Metabolic products
- Antigen detection
- Toxin detection
13
Q
Antibodies detection:
A
- Agglutination
- Precipitation
- CFT
- Neutralization
- IF
- ELISA
