med chem 23 Flashcards
chemical synthesis of peptides uses
- ester
- acid anhydride
-acid chlorides
biosynthesis of peptides uses
- esters ( active esters as w/ benzene group)
- acid anhydride
( we cant used acid chloride bc they r not found in nature and are very reactive )
acid anhydride are used as — in peptide biosynthesis and they are called – which are mixes of —
- intermediate
- mixed anhydride ( phosphoric and carbolyixic acid )
the main steps of ribosomal protein beiosystheis are the formation of amino acetyl-tRNA by :
then the peptide bond will be formed by:
involves the activation of an amino acid as anhydride
formed by reaction of aminoacetyl-tRNA with the growing peptide chain also attached to a t-RNA ( this involves amino group of one a.a with ester derative pf the growing peptide chain )
aminoacytl-AMP derative is a
mixed anhydride of phosphoric and carbolyxic acid
to form ester bond of aminoactyl-trna
we use:
to form amide bond in growing peptide we use:
- acid anyhydide
- ester ( ester is a part of the growing peptide in p-site and the incoming amino acid at a-site at the right side )
the sequence and assembly under nucleic acid control ( dna rRNA tRNA mRNA as 20L alpha amino acid refers to
ribosomal protein biosynthesis
refers to the sequence and assembly under control of a single enzyme ( without nucleic acid )
non-ribosomal peptide biosyethis as: more than 200 a.a identified to date
some natural peptides are sytheised by specific-multi functional enzymes called – and they consist of —
non- ribosomal peptide synthesis ( NRPS)
linear sequence of modules an each module insert a amino acid to the peptide sequence
Amino acids are first activated as —
aminoacytls-AMP dertaives are converted to —- by —— reaction
- anino-acytel-AMP derative
- thioesters by the reaction of thiol group of the NRPS
— are in ribosomal synthesis
— are in non-ribosomal synthesis
- ester
- thioester
