MBB11005 -Skills in Molecular Bioscience

Question 1

What is the volume range for a P10 pipette?

Answer 1

1-10μl

Question 2

What is the volume range for a P20 pipette?

Answer 2

2-20μl

Question 3

What is the volume range for a P200 pipette?

Answer 3

20-200μl

Question 4

What is the volume range for a P1000 pipette?

Answer 4

200-1000μl

Question 5

What is the volume range for a P5000 pipette?

Answer 5

1000-5000μl

Question 6

How is a slope culture prepared?

Answer 6

-tubes/small bottles of agar medium are heated to melt the medium and then left to cool/set in a sloped position
-inoculum is spread over the surface or applied in a streak with a sterile loop

Question 7

When is a slope culture used?

Answer 7

for culturing small amounts of bacteria
eg. to maintain large numbers of different cell lines in a small space

Question 8

How is a stab culture prepared?

Question 9

When is a stab culture used?

Question 10

How is a plate culture prepared?

Question 11

When is a plate culture used?

Question 12

How is a streak plate culture prepared?

Question 13

When is a streak plate culture used?

Question 14

How is a spread plate culture prepared?

Question 15

When is a spread plate culture used?

Question 16

How is a pour plate culture prepared?

Question 17

When is a pour plate culture used?

Question 18

How are broth cultures prepared?

Question 19

How are incubating cultures prepared?