Lecture 28 TCA Cycle

Question 1

What does anaplerotic reaction mean?

Answer 1

Replenishing

Question 2

What does the TCA constitute?

Answer 2

-it constitutes many biosynthetic pathways; maybe drawin away (Diverted,depleted) by cellular needs.
The intermediates must remain at a steady state for the cycle to continue at a steady state.

Question 3

What are the pathways that replenish TCA cycle intermidates called?

Answer 3

ANAPLEROTIC intermediates.

it serves an important function in maintaining metabolic fluxes.

Question 4

What is a malate aspartate shuttle?

Answer 4

NADH donation to ETC produces 3 ATP. The shuttle can function effectively only in cells ion which NADH/NAD+ ratio is much higher in cytosol than in mitochondrial matrix
o Shuttle NADH into mitochondria ONLY WHEN IT WAS CREATED BY GLYCOLYSI

Question 5

What does the anapleortic intermediates serve as?

Answer 5

Anaplerotic. They serve an important function in maintaining metabolic fluxes
	Pyruvate decarboxylase: oxaloacetate
	PEP carboxykinase: oxaloacetate
	PEP carboxylase: oxalocetate
	Malic enzyme: malate

Question 6

What are the two types of shuttles?

Answer 6

 Glycerol phosphate: FADH2 Donation 2 ATP
• Brugada Sydrome and SIDS: GPDH (glycerol3phosphate dehydrogenase defect lethal arrythmia
 Malate Aspartate: 3 ATP

Question 7

What is the stoichiometry of one TCA Cycle?

Answer 7

Glucose+ 10 (NAD+) + 4ADP +2H2O + 4pi +2FAD ===> 6CO2 +10 NADH + 4ATP + 2FADH2 + 6H+

Question 8

What are the porducts of TCA Cycle?

Answer 8

Per on glucose molecule, six carbons have been fully oxidized to CO2.