lecture 18 Flashcards
3 point mapping
what does recombination occur between?
heterozygous homozygous chromosomes
what are the genotypes involved in a test cross?
F1 generation crossed with a homozygous mutant
how do you identify the parental genotypes in 3 point mapping?
they are the two with the highest frequencies of the F2 generation
how do you find the expected percent of double recombinants?
multiply the RF between a-b by RF between b-c
how do you find the expected number of double recombinants?
multiply the expected percent of double recombinants by total number of offspring
what is the coefficient of confidence in regards to double recombination?
ratio of observed to expected double recombinants
how do you find the C.o.C.?
take the number of actually occurring double recombinants in the offspring and divide by the calculated number of expected recombinants
how do you find the interference in terms of double recombinants?
subtract C.o.C. from 1
what is occurring when one crossover event encourages the other crossover event to occur?
positive interference
is the interference coefficient positive or negative in positive interference?