lecture 11 microscopy Flashcards
what is microbiology
the study of all living organisms that are too small to be seen by the naked eye
what microbes are included in the study of micro b
bacteria, archaea, fungi, protozoa, algae, viruses, prions,
give the size range for each of these:
viruses
bacteria and archaea
eukaryotic microbes
viruses- 0.02-0.2 nm
bacteria and archaea- 0.2-2 nm
eukaryotic- 2-200 nm
what microorganisms can seen by the human eye
eukaryotic microbes
what are the major functions of a microscope
magnify and resolve and incrase contrast
what is the field of view
the diameter of the circle of light seen from a microscope
what is the depth of field
the thickness of a specimen that can be focused at a time
what kinnd of light microscopy is most seen by students
bright feild microscopy
what is the scientific name for bakers yeast
saccharomyces cerevisiae
give 3 limitations of bright feild microscopy
resolution, illumination, contrast
give 4 improvements od bright feild microscopy
oil immersion
dark feild
phase contact
differential interference contrast
what is bright field microscopy best for?
stained or naturally pigmented specimes
what is bright field microscopy useless for
live bacteria
whart is bright feild microscopy inferior for
non photosynthetic protists, metazoans, unstained cell suspensions, tissue sections
what does oil immersion do?
it reduces the amount od differacted light, which increses the amount going through the object
