Lec3

Question 1

identification of their nucleic acid genomes or proteins in viruses samples

Answer 1

directly detected

Question 2

by their pathological effects in cell culture or whole organisms, by identifying the presence of antibody that is specific for a known virus.

Answer 2

indirectly detection

Question 3

PCR products is detected by?

Answer 3

ethidium bromide staining of agarose gels

Question 4

are proteins produced by the immune system of higher vertebrates in response to foreign materials

Answer 4

Antibodies

Question 5

antibodies function?

Answer 5

have regions that recognize and bind specifically to the antigen.

Question 6

A serum that contains antibodies reactive against a particular entity

Answer 6

antiserum

Question 7

most widely-used method for viral protein detection is?

Answer 7

(ELISA)

Question 8

Detection of known viruses in clinical or other samples is the area of ?

Answer 8

viral diagnosis

Question 9

amplifies a specific DNA segment from a complex mixture of DNA molecules.

Answer 9

PCR

Question 10

PCR steps

Answer 10

1.denaturation
2.annealing
3.extension

Question 11

short DNA molecules called primers bind to regions of the target DNA

Answer 11

annealing

Question 12

double-stranded DNA templates are heated to separate the strands

Answer 12

denaturation

Question 13

DNA polymerase extends the 3′ end of each primer along the template strands. These steps are repeated (“cycled”) 25–35 times to exponentially produce exact copies of the target DNA

Answer 13

extension

Question 14

SYBR Green is a specific detection
T/F?

Answer 14

F, Non-specific detection

Question 15

Positive sense ssRNA viruses can be directly translated into protein
T/F?

Answer 15

T

Question 16

nucleic acid content of a virus has been used as?

Answer 16

a basis for classifying viruses.

Question 17

most widely-used method for viral protein detection

Answer 17

enzyme-linked immunosorbent assay (ELISA)

Question 18

PCR can’t be applied to detect viral DNA genomes in a sample
T/F?

Answer 18

F, can

Question 19

laboratory technique based on the polymerase chain reaction, enables both detection and quantification of a specific sequence in a DNA sample.

Answer 19

Real-time polymerase chain reaction/ (qPCR)

Question 20

according to the nature of the disease with which they are associated.

Answer 20

Classification on the basis of disease

Question 21

varicella zoster virus causes

Answer 21

chickenpox during a first infection

Question 22

knowing the shape of a virus particle does not allow us to predict anything about the biology, pathology or molecular biology of similarly-shaped viruses.
T/F?

Answer 22

T

Question 23

Who proposed a classification scheme which encompasses all viruses based on the nature of their genomes, and their modes of replication and gene expression.

Answer 23

David Baltimore

Question 24

David Baltimore classification are based on ?

Answer 24

1. nature of their genomes
2. modes of replication
3. gene expression.

Question 25

has established a taxonomic classification scheme for viruses.

Answer 25

(ICTV)

Question 26

assigning a virus to a taxonomic group characteristics.

Answer 26

1- Host range
2- Morphological features of the virion
3- Nature of the genome nucleic acid

Question 27

is a laboratory technique based on the polymerase chain reaction, enables both detection and quantification of a specific sequence in a DNA sample.

Answer 27

(qPCR)

Question 28

need RNA polymerase to form a positive sense RNA

Answer 28

• ssRNA Viruses

Question 29

viruses can be directly translated into protein

Answer 29

+ ssRNA