Lab Week 4 (Exam 2) Flashcards

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1
Q

What 2 basic capabilities should a microscope have?

A

1) magnification

2) resolution or resolving power

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2
Q

What is magnification?

A

How the big the image can become

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3
Q

What is resolution or resolving power?

A

How sharp the image is

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4
Q

When you increase magnification what 3 things happen?

A

1) image darkens
2) field of view decreases
3) depth of field reduces

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5
Q

What happens when the field of view decreases?

A

How much you can see all at once

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6
Q

What happens when the depth of field reduces?

A

How much you can see sharply at one time without adjustment

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7
Q

What 3 things can you do to correct the light source when the image is too dark?

A

1) turn up the lamp
2) system of lenses under stage and raise the condenser
3) in condenser there is iris diagram

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8
Q

What is a stage?

A

Platform where slide sits

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9
Q

What is the condenser?

A

System of lenses under the stage

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10
Q

What is an iris diaphragm?

A

It is a whole where the light passes through

Narrow it down to get a brighter image

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11
Q

What are the 2 lenses a microscope has?

A

1) eye piece or ocular (the place where you put your eyes)

2) nosepiece or turret (rotating piece above the stage

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12
Q

What is the objective lens?

A

All lenses on rotating piece (nosepiece or turret)

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13
Q

To figure out how magnified an image is what do you do?

A

Eyepiece x objective

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14
Q

What is high dry magnification and how much is that?

A

Highest you can get without using oil

400x

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15
Q

What is oil immersion lens and how much is it?

A

When you put the lens in oil to open light

Gives you the highest magnification

1000x

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16
Q

Where is the base of a microscope?

A

Bottom of microscope

17
Q

Where is the arm of a microscope?

A

Side of microscope where you carry it

18
Q

Where and what is the lamp?

A

Under stage

Gives light

19
Q

What is the mechanical stage?

A

Wheels operating to position slide

20
Q

What is the coarse adjustment?

A

Moves stage up and down and used to focus image with lower magnification

21
Q

What is the fine or focus adjustment?

A

Moves stage up and down very little and is used to focus image with a higher magnification

22
Q

What is a stain?

A

Technique used to enable better visualization of cells and cell parts under the microscope