Lab Questions

Question 1

bla (selectable marker)

Answer 1

antibiotic resistance, kills bacteria without plasmid

Question 2

origin of replication

Answer 2

allows the plasmid to be copied on its own

Question 3

GFP

Answer 3

glowing gene, gene of interest

Question 4

araC

Answer 4

repressor protein, binds to promoter pBAD, stops transcription, arabinose allows transcription

Question 5

Why can we separate GFP by HIC Chromatography?

Answer 5

HIC Chromatography allows proteins to be separated on whether they are hydrophobic or not. Since GFP is hydrophobic, it’s able to easily bind to its column and separate itself from the rest of the hydrophilic proteins.

Question 6

aseptic technique

Answer 6

process of keeping bacteria in the lab

Question 7

heat shock

Answer 7

cooling bacteria, heat it up fast, then cool it down again

Question 8

+ LB/Amp

Answer 8

grow

Question 9

+ LB/Amp/Ara

Answer 9

glow and grow

Question 10

• LB/Amp

Answer 10

nothing occurs

Question 11

• LB

Answer 11

grow

Question 12

• LB/Ara

Answer 12

grow

Question 13

+ LB/Ara

Answer 13

grow and glow

Question 14

• LB/Amp/Ara

Answer 14

nothing occurs