Lab exam one-ex. 4 Flashcards
1
Q
PAGE
A
technique to separate proteins in a mixture in order to identify tyrosinase
2
Q
native gels
A
gel solutions and electrophoresis temperatures are carefully chose to retain the native conformation
3
Q
denatured gel
A
conditions are used to destroy the native conformation and activity of the protein
4
Q
SDS
A
added to remove molecular charge as a factor in movement of proteins through the electrophoretic field
5
Q
stacking gel
A
top component, more porous than resolving gel and causes the proteins to stack at the interface
