LAB Chapter 2 (microscope)

Question 1

How do you see the image in the microscope?

Answer 1

Up side down and reverse

Question 2

If you move the slide on the stage how will it appear to move to you?

Answer 2

If you move the slide toward you it will move away if you move the slide to the left it will move to the right. It moves backwards visually from how you move it physically

Question 3

The two parts of the lenses that allow you to see the image

Answer 3

The ocular eyepiece in the objective lenses.

Question 4

What is the total magnification TM

Answer 4

Ocular eyepiece is 10 X which is then multiplied to whatever magnification the objective lens has
Example If you have a 40 X objective lens it would be 40×10 which is 400X magnification

Question 5

Using high power lens what are the two things that are different in the image as compared to low power lens

Answer 5

1 image is much larger

2 I can see more detail

Question 6

What happens to the depth of field as you move higher in magnification

Answer 6

The depth of field decreases

Question 7

What is the base

Answer 7

Supports the microscope and holds a light source

Question 8

What is the arm of the microscope

Answer 8

It’s the part of the microscope that extends from the base and supports the ocular lens

Question 9

What is the mechanical stage

Answer 9

Holds the specimen mounted on the slide.

Question 10

What is the lamp on a microscope?

Answer 10

It is a light source that illuminates the slide from underneath

Question 11

Explain the lenses and where they are located with the names

Answer 11

Ocular lenses or what you look through And have a 10x magnification We are located at the top of the arm
Ejective lenses have a magnification of 10, 40 and 100 X. We are located at the bottom of the nose piece and rotates to be used just above the specimen

Question 12

Where is the condenser and what does it do

Answer 12

Located directly above the light source it concentrates the light on the specimen

Question 13

Where is the iris diaphragm and what does it do

Answer 13

attached to the condenser that regulates the amount of light passing through the condenser

Question 14

Where is the course adjustment in the fine adjustment knob and what do they do?

Answer 14

Located on the other side just above the base below the stage. Course adjustment raises and lowers the mechanical stage rapidly to assist in focus. Fine adjustment raises and lowers it too much lesser degree assisting in fine focusing of the Image

Question 15

What is resolution

Answer 15

The ability to discern the details of the image

Question 16

What is depth of field

Answer 16

The thickness of the specimen that can be clearly seen at any one time

Question 17

Why is Stain used

Answer 17

It’s used to provide a contrasting media so Individual parts of the cell can be seen

Question 18

How does the amount of light change when switching from a low to a high power objective lens

Answer 18

The light decreases as the magnification increases

Question 19

What happens to the depth of focus change as you move higher in magnification

Answer 19

The depth of focus is reduced as the magnification increases