Lab 4 - Bradford Assay and BCA Assay Flashcards
1
Q
Purpose of Bradford Assay
A
Measures the concentration of protein in a solution
2
Q
Purpose of BCA Assay
A
Determines the total concentration of protein in a solution (mg/mL or micrograms/mL)
3
Q
What molecule was used as a standard for the Bradford and BCA Assays?
A
BSA protein
4
Q
Assay graph
A
x-axis = Concentration (mg?) y-axis = Abs @ \_\_\_\_ nm
5
Q
Similarities between Bradford and BCA
A
Come back to
6
Q
Differences between Bradford and BCA
A
Come back to
7
Q
What is the name of the machine we measured the results of the assay with?
A
Spectrophotometer
