Krebs cycle Flashcards
things we need to add?
water to first to all dehydrogensase NADH + H+ --> NAD+ NADH + H+ --> NAD+ FADH2 --> FAD NADH + H+ --> NAD+
8 reactions
condensation isomerisation decarboxylation oxidative decarboxylation substrate level phosphorylation dehydrogenation hydration dehydrogenation
importance of condensation reaction
thioester bond cleaved; hence v exergonic
importance of isomerisation
endergonic, but high conc of citrate drives it forward
importance of oxidative decarboxylation
enzyme - alpha-ketoglutarate dehydrgonase complex - is homologous to pyruvate dehydrogenase, but different substrate specificity
importance of substrate level phosphorulation
thioester bond cleaved; v exergonic so drives GTP formation
GDP GTP delta G=0
importance of dehydrogenation
succinate dehydrogenase embedded in inner mito memb and has covalently attached FAD for entry of eelectrons into it
importance of hydration
malonate is a structural analogue of succinate so can hinibit it
importance of dehydrogenation
v endergonic but driven forward by low oxalocatetate conc
oxaloacetate conc kept low by endergonic citrate fromation
