Krebs cycle

Question 1

things we need to add?

Answer 1

water to first
to all dehydrogensase
NADH + H+ --> NAD+
NADH + H+ --> NAD+
FADH2 --> FAD
NADH + H+ --> NAD+

Question 2

8 reactions

Answer 2

condensation
isomerisation
decarboxylation
oxidative decarboxylation
substrate level phosphorylation
dehydrogenation
hydration 
dehydrogenation

Question 3

importance of condensation reaction

Answer 3

thioester bond cleaved; hence v exergonic

Question 4

importance of isomerisation

Answer 4

endergonic, but high conc of citrate drives it forward

Question 5

importance of oxidative decarboxylation

Answer 5

enzyme - alpha-ketoglutarate dehydrgonase complex - is homologous to pyruvate dehydrogenase, but different substrate specificity

Question 6

importance of substrate level phosphorulation

Answer 6

thioester bond cleaved; v exergonic so drives GTP formation

GDP GTP delta G=0

Question 7

importance of dehydrogenation

Answer 7

succinate dehydrogenase embedded in inner mito memb and has covalently attached FAD for entry of eelectrons into it

Question 8

importance of hydration

Answer 8

malonate is a structural analogue of succinate so can hinibit it

Question 9

importance of dehydrogenation

Answer 9

v endergonic but driven forward by low oxalocatetate conc

oxaloacetate conc kept low by endergonic citrate fromation