in class questions for exam 2

Question 1

supercoiling in bacteria is typically introduced by an enzyme called

Answer 1

DNA gyrase

Question 2

what is the correct order of proteins involved in protein replication

Answer 2

1. DNAa
2. Primase
3. DNA poly 3
4. Ligase

Question 3

where does RNA priming occur?

Answer 3

3’ hydroxyl group that adds nucleotides

Question 4

the sigma factor is directly required for what?

Answer 4

transcription initiation

Question 5

termination of transcription can be caused by what two things?

Answer 5

Rho factor or hair pin loop

Question 6

if the genetic code was in quadruplets instead of triplets there would be how many codons?

Answer 6

4^4 256
4^3 64 (original)

Question 7

peptidyl transferase catalyzes the movement of the peptide from the transfer RNA in the —– to the transfer RNA in the —–

Answer 7

P site to A site

Question 8

chaperones are proteins that do what?

Answer 8

help proteins fold

Question 9

where are the signal sequences of a protein (polypeptide)?

Answer 9

the N-terminus

Question 10

similar genes within the same organism but have a different functions are called what?

Answer 10

paralogs

Question 11

(T or F) Rho termination of transcription requires a hairpin loop

Answer 11

False

Question 12

(T or F) periplasmic proteins are secreted by ATP-dependent proteases

Answer 12

False: periplasmic proteins are secreted by secA