Immunology & Serology Flashcards
CD8 surface marker
Class I - Cytotoxic T cell
CD 4 surface marker
Class II - Helper T cell
antigen receptors on T lymphocytes bind HLA class II molecules with the help of which accessory molecule?
CD4
Most common anticoagulant in Apharesis
ACD
Expiry:
CPD, ACD, CP2D
21 days
Expiry:
CPDA-1
35 days
Expiry:
Additional solutions
42 days
Additional solutions
AS1:
AS3:
AS5:
AS1: Adsol
AS3: Nutricel
AS5: Optisol
AHG control cells are coated with
IgG antibodies
True or false?
AHG could be use for both DAT and IAT.
True
AHG is positive control for ____ reagents
Anti-IgG
AHG must be used to confirm all ______ antiglobulin reactions
negative
Cytotoxic T cells are usually CD_
CD8
Surface marker for mature T cells
CD2
_____ used in the identification of T cells where a T cells CD2 surface protein is bound to a sugar based LFA-3 homologue on the surface of a sheep red blood cell
E-rosetting
The term kernicterus means increased ______.
Unconjugated bilirubin
Kernicterus aka
Bilirubin encephalopathy
Kernicterus shows >__ mg/dL of unconjugated bilirubin among infants with HDN
20
*causes acidosis and hypoalbuminuria
What cell produces antibody?
Plasma cells
Marker for plasma cells
CD38 & CD 138, CD27++
Marker for Naive B cells
CD27-
Marker for memory B cells
CD27+
PCR technology can be used to ______ DNA.
Amplify small amounts of DNA
Class 1 MHC presents antigen to
CD8 + T lypmhocytes
Old Pan T
CD2 + T lymphocytes
New Pan T
CD3 + T lymphocytes
Whole blood collection procedure
If the volume collected is in the low volume range (300 to 404 mL in a 50 mL collection or 333 to 449 mL in a 500 mL collection), the unit must be labeled as a “________,” and fresh frozen plasma (FFP) cannot be made from this unit, as it would not contain adequate levels of coagulation factors.
low volume unit
Parenterally means acquired from ____
blood
Primarily transmitted parenterally
HGV (most)
HDV
HBV
HCV
Disinfectant for BB blood collection
Iodine
If patient is allergic to iodine use ______.
Chlorhexidine gluconate + Isopropyl alcohol
In Blood donation collection, scrub in all direction about ___cm for ___ seconds.
4 cm
30 secs
Blood donation collection
gauge:
angle:
Anticoagulant volume:
Ag to blood ratio:
gauge: 16
angle: 20°
Anticoagulant volume: 63 mL
Ag to blood ratio: 1:7
Formula for the adjusted volume of blood to be collected and anticoagulant to be used.
Volume to collect:
Reduced volume of anticoagulant:
Amount of solution to be removed:
Volume to collect: (donor’s weight in Kg/50) x 50 mL
Reduced volume of anticoagulant: (Volume to collect/450) x 63mL
Amount of solution to be removed: 63 mL - calculated volume
T or F?
Antigen-antibody reactions are reversible?
True
When may an IS crossmatch be performed?
When there is no history of antibodies and the current antibody screen is negative.
Antibody idiotype is dictated by the: (BOC)
Variable regions of heavy and light chains
Which is TRUE about Anamnestic response?
a. Species specificity
b. Gradual increase in titer
c. First encounter with the antigen
d. Subsequent exposure to that same antigen
D
All of the following are carcinogenic, EXCEPT
a. asbestos
b. radiation
c. organic herb
C
