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HDS > Histology Stains > Flashcards

Histology Stains Flashcards

1
Q

Eosin

A

Proteins, cytoplasm, SER, collagen
Pink (collagen, proteins, cytoplasm)
Red-pink (SER)
With hematoxylin

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2
Q

Trichrome

A

RBCs, Muscle, CT
RBCs – orange
Muscle – red
CT – blue, green

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3
Q

Periodic Acid-Schiff (PAS)

A

Carbs such as glycoproteins or proteoglycans
Magenta, bright pink
Stains glycocalyx, basement membrane

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4
Q

Toluidine/Methylene/Alcian Blue

A

Neg charged groups (phosphates, sulfates)
Carboxylic rich mucopolysaccharides
Neg charged groups stain purple (blue background)
Stains phosphorylated or sulfated molec in cells or EC matrix purple

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5
Q

Silver chloride

A

Reticular (Type II) collagen, Neurofibrils

Black

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6
Q

Elastic fiber stains

A

Elastic fibers

Blue-black or magenta (depending on stain)

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7
Q

Osmium

A

Unsaturated lipids

Black or brown

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8
Q

Hematoxylin

A

Nucleic Acids, nucleus and ribosomes
Purple/blue
With eosin

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9
Q

Masson’s Trichrome

A

Collagen fibrils: bright blue
Nuclei: dark purple, blue-black
Cytoplasm, calcified collagen, mucle: dark pink

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10
Q

VerHoeff’s (Orcein) Stain

A

Elastic fibers: dark purple/reddish brown

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11
Q

Fluorophores

A

absorv UV light and emit light at a lower excitation level/wavelength in the visible spectrum
fluorophores chemically conjugated with secondary antibodies

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12
Q

DAPI

A

DNA-intercalcating agent

Used in conjugated antibodies with fluorescent microscopy to tag NUCLEUS

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13
Q

Hematoxylin

A 
Nucleic acids
Nucleus
Ribosomes
RER
Purple/blue
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14
Q

Ammoniacal silver

A

golgi
reticular fibers
neurofibrils
black

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15
Q

Osmium reduction

A

unsat fatty deposits

black to dark brown

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16
Q

Elastic tissue stain

A

elastic fibers

blue-black

17
Q

Gill’s H & E (stains used; acidic or basic?)

A 
hematoxylin (basic)
acid fuchsin (acidic)
xylindine (basic)
eosin Y (acidic)
fast green (acidic)

HDS (14 decks)

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