Histology Flashcards
What does alcian blue dye? What colour?
Alcian blue dyes GAG-rich structures, mucous goblet cells, mast cell granules and cartilage matrixes BLUE.
What does eosin dye? What colour?
Colloidal proteins (eg plasma) - PINK Keratin - ORANGE/RED
What does Iron Haematoxylin dye? What colour?
Nuclei, Elastic fibres - BLACK
What does haematoxylin dye? What colour?
Nuceli, RNA - Blue
What does Periodic Acid Schiff (PAS) dye? What colour?
Hexose sugars (especially those contained in complex carbohydrate containing structures including goblet cell mucins, cartilage matrix, glycogen, basement membranes, glycocalyx) - MAGENTA (DARK PINK)
What does Romanovsky Stains (eg, giemsa’s of leishman’s stains for blood films) dye? What colour?
Chromatin, (nuclei), azurophils, neutrophil granules - PURPLE
Erythrocytes - RED/PINK
Eosinophil granules - PALE BLUE
Lymphocyte and monocyte cytoplasm, basophil granules - DARK BLUE/PURPLE
What does Toluidine Blue dye? What colour?
Nuclei, ribosomes, cytoplasm - DARK BLUE
Cartilage matrix, - PALE BLUE
mast cells granules, GAG rich components - BRIGHT PURPLE
(DNA and RNA rich, cancers due to binding of DNA dividing cells, also used for sexual assault as it stains damaged tissues more)
What does Van Gieson’s trichrome stain (with haematoxylin counter-stain) dye? What colour?
Van Gieson’s trichrome stain (with haematoxylin counter-stain) dyes
Collagen - PINK/RED
Cell cytoplasm - YELLOW/OLIVE GREEN
Nuclei - BLACK
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What does Eosin dye? What colour?
Eosin dyes collodial proteins (eg plasma) PINK
and keratin - ORANGE/RED
What does iron haematoxylin dye? What colour? (not routinely used in diagnostics)
Iron haematoxylin dye nuclei and elastic fibres BLACK
What does Haematoxylin dye? What colour?
Haematoxylin dyes nuclei and RNA BLUE
What does Periodic Acid Schiff (PAS) dye? What colour?
Periodic Acid Schiff (PAS) dyes Hexose sugars (especially those contained in complex carbohydrates containing structures including goblet cell mucins, cartilage matrix, glycogen, basement membranes, glycocalyx) MAGENTA (dark pink)
What do Romanovsky strains (eg, giemsa’s of leishman’s stains for blood films) dye? What colour?
Chromatin, (nuclei), azurophils, neutrophils, granules - PURPLE
Erythrocytes - RED/PINK
Eosinophil granules - PALE BLUE
Lymphocyte and monocyte cytoplasm, basophil granules - DARK BLUE/PURPLE
What does Toluidine Blue dye? What colour?
Nuclei, ribosomes, cytoplasm - DARK BLUE
Cartilage matrix, mast cell granules - PALE BLUE
GAG rich components - BRIGHT PURPLE
