Gram Positive Cocci

Question 1

Catalses Positive

Answer 1

Staphylococcus

Question 2

Catalses Negative

Answer 2

Streptococcus & Enterococcus

Question 3

Coagulase Positive

Answer 3

S. aureus

Question 4

Cogaulase Negative

Answer 4

Other Staphylococcus spp.

Question 5

Staphylococcus aureus

Answer 5

AST - MRSA
Screening test - Mannitol Salt + CHROMagar
Hemolytic - Beta
PCR for mecA and PTP 2A

Question 6

Other Staph spp.

Answer 6

AST - Novobiocin Resistances testing

Modified oxidase Positive

Question 7

Streptococcus spp.

Answer 7

S. pyogenes
S. agalactiae
S. pnuemonia
S. virdians

Question 8

Beta Hemolytic

Answer 8

PYR test / CAMP Test / Hippurate Test
S. pyogenes - PYR Positive
S. agalactiae - CAMP & Hippurate Positive

Question 9

Alpha Hemolytic

Answer 9

Optochin / Bile Solubility
S. pnuemonia - Optochin susceptible + Bile Solubile Neg
S. virdians - Optochin resistant + Bile Soluable Pos

Question 10

Enterococcus

Answer 10

PYR Test / Bile Esculin test
E. faecalis & E. faecium
PYR & Bile Esculin Positive
Halophilic - grows in 6.5% media