Glycolysis2 Flashcards

1
Q

What enzyme cleaves off the fructose 1,6-bisphosphate molecule?

A

Aldolase

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2
Q

What enzyme converts the Dihydroxyacetone phosphate molecule to G3P?

A

Triose-Phosphate isomerase

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3
Q

What enzyme dehydrogenates the G3P molecule?

A

Glyceraldehyde 3 phosphate dehydrogenase

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4
Q

At what step of glycolysis does the first substrate level ATP forms?

A

7th step from the conversion of 1,3BPG by PG kinase

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5
Q

What happens at the 8th step of glycolysis?

A

Phosphoglycerate Mutase converts the 3-PG to 2-PG (This is not isomerization)

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6
Q

What is the Function of enolase enzyme in the glycolysis process?

A

It dehydrogenates the 2-PG to phosphoenolpyruvate molecule

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7
Q

At what step does the second substrate level ATP phosphorylation takes place?

A

At the 10th step during conversion of PP to pyruvate

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8
Q

At what steps of glycolysis are ATP used?

A

1st and 3rd

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9
Q

Why does the G6P to F6P isomerization happens?

A

Due to presence of carbonyl compound on the 1st carbon of glucose. Aldose converted to Ketose compound

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10
Q

What are the irreversible reactions of glycolysis?

A

All are reversible except the 1st, 3rd and 10th steps

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11
Q

What are the two phases of glycolysis?

A

Preparatory and payoff phases

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12
Q

How many ATPs are produced and used in glycolysis?

A

4ATPS are produced whereas 2ATPs are consumed hence net ATP produced is 2

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13
Q

What is the NAD+ reducing stage of glycolysis?

A

6th step when G3P is being converted to 1,3-BPG

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14
Q

How many NADHs are produced at glycolysis level?

A

2

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15
Q

At what stage is water produced in glycolysis?

A

During conversion of 2-PG to Phosphoenolpyruvate

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16
Q

G3P gets converted to 1,3-BGP but interestingly no ATPs are consumed? Justify!

A

Because this process uses the inorganic phosphate from cytosol

17
Q

What are the three fates of pyruvate?

A

2 fermentations( microbial or muscular) in absence of oxygen and oxidative phosphorylation during aerobic conditions.