Exercise 6: Pure cultures: Preparing streak plates Flashcards
1
Q
What is a pure culture?
A
A pure culture is a group of bacteria that is of one species.
2
Q
What is the most common way to sterilize medium?
A
The most common way to sterilize medium is by autoclaving (steam under pressure) at a temperature of 121oC at 15 lbs of pressure/sq. in. for a period of 15 minutes.
3
Q
What are streak plates used for?
A
Common way to obtain a pure culture.
4
Q
How do you know that you’ve created a proper streak plate.
A
To properly streak a plate for isolation means spreading out the organism(s) by means of the inoculating loop, or thinning the culture, until single colonies result.
5
Q
What is heavy inoculum?
A
Bacterial growth should completely cover this area after incubation.
