Epithelium Flashcards

1
Q

Eosin stain

A

PINK, Acidic dye, Na + radical

Stains cytoplasm, cytoplasmic fiaments, collagen fibers, basement membrane

Stained objects = “acidophilic” or “eosinophilic”

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2
Q

Hematoxylin Stain

A

PURPLE, Basic dye, Cl- radical

Stains heterochromatin, nucleolus, RER, sulfated GAGs

Objects stained = basophilic

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3
Q

Identify

A

Simple squamous

Location: lining of vessels, vacities, pericardium, pleura, peritoneum

Fx: Movement of viscera, active transport by pinocytosis, secretion of biologically active molecules

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4
Q

Identify

A

Simple Cuboidal

Location: Covering the ovary and thyroid

Fx: Covering and secretion

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5
Q

Identify

A

Simple columnar

Location: Lining of intestine and gallbladder

Fx: Protection, lubrication, absorption, secretion

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6
Q

Identify

A

Pseudostratified (columnar/cuboidal)

Location: lining of trachea, bronchi, nasal cavity

Fx: Protection, secretion, cilia-mediated transport of particles in mucus

(picture is columnar and ciliated)

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7
Q

Layers of Epidermis

A

Stratum basale
Stratum spinosum
Stratum granulosum (with keratohyalin granules)
Stratum lucidum (clear, only in thick skin)
Stratum corneum

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8
Q

Melanocytes

A

Neural crest origin

Melanin granules passed to keratinocyte. Number and distribution of granules in keratinocytes determines skin color

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9
Q

Langerhans Cells

A

Antigen presenting cells from bone marrow
2-8% of epidermal cells
Unique organelle in TEM - Birbeck granule

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10
Q

Merkel Cells

A

Found mainly in stratum basale of thick skin
Sensitive mechanoreceptor
Merkel cell carcinomas are rare but hard to treat

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11
Q

Basement membrane

A
Basement Membrane (LM) is the Basal Lamina (TEM) plus the Reticular Lamina (TEM)
Examples of basement membranes: respiratory epithelium, glomerular basement membrane
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12
Q

Types of cells in simple columnar epithelium

A
  1. Goblet cells: mucus producing, pale/clear cytoplasm
  2. Enterocytes: pink, elongated, purple basal nucleus
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13
Q

Identify

A

Simple cuboidal epithelium surrounding follices from the thyroid.

Arrow points to blood vessels

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14
Q

Identify

A

Simple columnar epithelium lining the villi

From the ileum

Goblet cells (clear cytoplasm)

Enterocytes (boxed)

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15
Q

Identify

A

Stratified squamous epithelium (top)

Connective tissue (bottom)

Boundary: basal lamina/basement membrane

From esophagus

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16
Q

Identify

A

Stratified Squamous Keratinized Epithelium (Thick Skin)

From Sole of Foot

Pink = keratinized layer

Purple = epidermis (see pegs)

Squames

17
Q

Identify

A

Pseudostratified Columnar Ciliated Epithelium (aka respiratory epithelium)

From Human Trachea

Thick basement membrane (pink)

Connective tissue with glands and rings made of hyaline cartilage

18
Q

Identify

A

Pseudostratified Columnar Ciliated Epithelium from Human Trachea

Goblet cells, ciliated cells, lymphocytes (black dots), basal bodies (dark pink line at base of cilia)

Thick basement membrane

19
Q

Identify

A

Transitional Epithelium from Human Bladder (relaxed)

H & Chromotrope staining

Epithelium just the dark purple later at top

20
Q

Identify

A

Transitional epithelium

Binucleated “dome” cells (cuticle above nuclei)

Pseduostratified epithlium

Thin basement membrane

Connective tissue

21
Q

Identify

A

Transitional epithelium of distended bladder

Epithelial layer is thinner

Dome cells on lumen side, squamous in shape

22
Q
A
23
Q

Define stereocilia

A

Not normal cilia because they aren’t motile, more like microvilli but longer.

Found on apical surface of some cells

24
Q

Define striated border

A

Microvilli and glycocalyx of small intestines

25
Q

What is the response of melanocytes to UV raditation

A

Tanning darkens melanin and accelerates melanin synthesis

26
Q

Junctions on lateral membrane of intestinal epithelial cell

A

Gap junctions

Desmosomes

Zonula occludens (tight jxn)

Zonula adherens

27
Q

3 types of microvilli and examples

A
  1. Striated border (small intestine)
  2. Brush border (proximal renal tubule)
  3. Stereocilia (epididymis and ductus deferens of male repro)
28
Q
A
29
Q

What layers of epidermis have proliferating keratinocytes

A

Stratum basale and spinosum

30
Q
A
31
Q

Differences between thin and thick skin

A

Distinguished by thickness of epidermal layer

Thick skin is hairless, only on soles of feet and palms

Thin skin doesn’t have a stratum lucidum (clear layer)