Enzyme Kinetics Flashcards
What does tyrosinase form when tyrosine is a substrate?
Dopaquinone
What does dopaquinone spontaneously form into?
Dopachrome
What color is dopachrome and where is it found?
Red-orange pigment found in the hair of red-heads
What is dopachrome an intermediate to the production of?
Black-brown pigment melanin
What kind of reaction foes tyrosinase catalyze?
Oxidation
How is the enzyme activity measured?
By monitoring the appearance of dopachrome at a wavelength of 475 nm
What substrate is used in the experiment?
L-DOPA
What are we determining the rate of in the experiment?
The rate of formation of dopachrome (from L-DOPA substrate)
Tyrosinase exhibits typical Michaelis-Menton kinetics . T or F?
True
What kind of reaction does tyrosinase catalyze when L-DOPA is the substrate?
Oxidative decarboxylation
What is L-DOPA sometimes used in the treatment of?
Parkinson’s disease
What was sodium phosphate used for in the experiment?
A buffer
What was used as the inhibitor in the experiment?
Sodium benzoate
Which component of the experiment is kept constant?
The amount of enzyme
Which component of the experiment was manipulated?
The substrate
