ELISA

Question 1

Draw an elisa to detect an antigen.

Answer 1

the antibody should be bound to the bottom of the well, antibodies and antigens will be added, the well should be washed, the antigen will bind to the antibody, conjugate (enzyme labeled) will be added, it should be washed again, substrate should be added, if positive the color will change

Question 2

Draw an elisa to detect an antibody.

Answer 2

well is pre-coated with antigen, test serum is added (antibody), enzyme-labeled antigen is added, substrate is added and color should change

Question 3

When will a precipitate occur in a precipitate assay?

Answer 3

when there are optimal (equal) amounts of both antigen and antibody

Question 4

When will a precipitate not occur in a precipitation assay?

Answer 4

when there are unequal amounts of antigen or antibody; more antigen to antibody or more antibody to antigen

Question 5

Define titer.

Answer 5

last positive dilution during a titration that tests for antibody presence in a serum

Question 6

What is titration?

Answer 6

The dilution of a serum to determine the amount of antibody in that serum