DNA replication Flashcards
DNA helicase
enzyme that unzips the DNA
DNA polymerase
enzyme that connects a strand of DNA to another to form a complete helix
replication fork
the ends of each replication bubble
replication bubble
the origins of the DNA helicase unzipping the DNA
prokaryotes form how many rep. bubbles?
one
eukaryotes form how many rep. bubbles?
multiple
semiconservative model of replication
separated strands act as templates for a new complementary strand to form two DNA helix’s
DNA polymerase can only add new nucleotides to the
3’ ends- strands will be built in a 5’ to 3’ direction
prokaryotic DNA rep.
since the strand is like a ring there is only one bubble that goes in two directions
which enzyme proofreads DNA
Exonuclease and polymerase
how many mistakes are made in base pairs?
1 in 10000
how many mistakes in base pairs after correction?
1 in 1 billion
what damage DNA?
chemicals and radiation
excision repair
when any of over 50 repair enzymes remove damages pairs of DNA
role of DNA ligase and polymerase in excision repair
they insert and bond the new nucleotides together after the damaged DNA is taken out