DNA repair/recomb continued

Question 1

An ____ test uses a selectable marker that can give a readily observable _____ (such as growth on antibiotic) when _____ happens.

Answer 1

• Ames
• phenotype
• mutation

Question 2

Ames test:
By comparing the number of cells with the observable ____ in the presence of a ___ ____ to the number of cells in another tube ___ that compound, the _____ of a compound can be determined.

Answer 2

• phenotype
• test compound
• lacking
• mutagenicity

Question 3

Recombination of DNA results in ___ __ ____ of DNA sequences.

Answer 3

-mixing and matching

Question 4

The process of DNA recombination occurs most often between ____ ____ on ____ chromosomes. The process can be quite ____ during _____.

Answer 4

• homologous sequences
• different
• active
• meiosis

Question 5

Recombination proceeds through formation of ____ ____ as a result of alignment of _____ _____.

Answer 5

• Holliday junctions

- homologous sequences

Question 6

Alignment is followed by cleavage of strands on each chromosome, invasion of the strands into the opposite chromosome.

Answer 6

• cleavage of stands
• invasion
• opposite

Question 7

After invasion, there is ____ of the ___, another _____ reaction, followed by _____ of _____ _____.

Answer 7

• movement
• junction
• cleavage
• reformation
• phosphodiester bonds

Question 8

Enzymes involved in recombination are called ____ and are similar in function to the ____ of ___.

Answer 8

• recombinases
• integrase
• HIV

Question 9

Error-related systems associated with cancer include _____ (___ cancer) and ____ (___ cancer), which is involved in DNA ____. A critical protein for monitoring DNA for ____ prior to division is ____.

Answer 9

• HNPCC (colon cancer)
• BRC-A (breast cancer)
• repair
• damage
• p53